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pLEX307-SARS-CoV-3CL
(Plasmid #160280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 160280 Standard format: Plasmid sent in bacteria as agar stab 1 $75

Currently unavailable outside the U.S.

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLEX_307
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable ; NEB 10Beta
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3CL Protease
  • Species
    SARS-CoV
  • Insert Size (bp)
    924
  • Promoter EF1a

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX307-SARS-CoV-3CL was a gift from Alejandro Chavez (Addgene plasmid # 160280 ; http://n2t.net/addgene:160280 ; RRID:Addgene_160280)
  • For your References section:

    A simplified cell-based assay to identify coronavirus 3CL protease inhibitors. Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Nair MS, Huang Y, Tay NES, Rovis T, Yang HW, Stockwell BR, Ho DD, Chavez A. bioRxiv. 2020 Aug 29. doi: 10.1101/2020.08.29.272864. 10.1101/2020.08.29.272864 PubMed 32869020