pVRC8400-SARS-CoV-2-S2P-AVI
(Plasmid
#160474)
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PurposeExpresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 160474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | ||
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepVRC8400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSARS-CoV-2-S2P
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Tags
/ Fusion Proteins
- scFc (N terminal on insert)
- HRV3C (N terminal on insert)
- AVI (C terminal on insert)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVRC8400-SARS-CoV-2-S2P-AVI was a gift from Peter Kwong (Addgene plasmid # 160474 ; http://n2t.net/addgene:160474 ; RRID:Addgene_160474) -
For your References section:
Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes. Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Lopez Acevedo SN, Madan B, Olivera de Souza M, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang L, Abiona O, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L. SSRN. 2020 Jul 21:3639618. doi: 10.2139/ssrn.3639618. 10.2139/ssrn.3639618 PubMed 32742241
