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Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.
Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Lopez Acevedo SN, Madan B, Olivera de Souza M, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang L, Abiona O, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L
SSRN. 2020 Jul 21:3639618. doi: 10.2139/ssrn.3639618. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160474pVRC8400-SARS-CoV-2-S2P-AVIExpresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160475pVRC8400-SARS-CoV-2-NTD-AVIExpresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160476pVRC8400-SARS-CoV-2-RBD-AVIExpresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160477pVRC8400-SARS-CoV-2-RBD-SD1-AVIExpresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160478pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVIExpresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160479pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVIExpresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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160480pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVIExpresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
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