Addgene - pEGFP-C1 beta-catenin Y654F Plasmid Data

Plasmid 16072: pEGFP-C1 beta-catenin Y654F

Gene/insert name:
beta-catenin
Insert size:
2345
Species:
G. gallus (chicken)
Entrez Gene:
CTNNB1 (CHBCAT)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Mutation:
Y654F
Vector backbone:
pEGFP-C1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
BspE1/BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
EGFP-C List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Erin Schuman
Terms and Licenses:
MTA

Comments:

Phosphorylation-prevented mutant of beta catenin.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function. Murase et al (Neuron. 2002 Jul 3. 35(1):91-105. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16072" in your Materials and Methods section.