pEGFP-C1 beta-catenin Y654F
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16072||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesG. gallus (chicken)
Insert Size (bp)2345
MutationY654F, *(see below)
Entrez GeneCTNNB1 (a.k.a. CHBCAT)
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BspE1/BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Phosphorylation-prevented mutant of beta catenin.
*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-C1 beta-catenin Y654F was a gift from Erin Schuman (Addgene plasmid # 16072 ; http://n2t.net/addgene:16072 ; RRID:Addgene_16072)
For your References section:Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function. Murase S, Mosser E, Schuman EM. Neuron. 2002 Jul 3. 35(1):91-105. 10.1016/S0896-6273(02)00764-X PubMed 12123611