pEGFP-C1 beta-catenin WT
(Plasmid #16071)

• Depositing Lab: Erin Schuman
• Publication: Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin
• Species: G. gallus (chicken)
• Insert Size (bp): 2345
• Mutation: *
• Entrez Gene: CTNNB1 (a.k.a. CHBCAT)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspE1/BamHI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that this plasmid--and all of the beta-catenin plasmids associated with this paper--have L95R and S96A mutations relative to the canonical WT. Addgene believes these are polymorphisms and that our stock is representative of what was used in the associated paper. Additionally, we see G636A