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lentiCRISPR v2-sgSLC7A11/xCT-2
(Plasmid #161819)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161819 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone manufacturer
    Feng Zhang
  • Backbone size w/o insert (bp) 12000
  • Total vector size (bp) 12000
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    solute carrier family 7 member 11
  • Alt name
    SLC7A11
  • Alt name
    xCT
  • gRNA/shRNA sequence
    AAGTATTACGCGGTTGCCAC
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_014331.4
  • Entrez Gene
    SLC7A11 (a.k.a. CCBR1, xCT)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiCRISPR v2-sgSLC7A11/xCT-2 was a gift from Boyi Gan (Addgene plasmid # 161819 ; http://n2t.net/addgene:161819 ; RRID:Addgene_161819)
  • For your References section:

    Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. Liu X, Olszewski K, Zhang Y, Lim EW, Shi J, Zhang X, Zhang J, Lee H, Koppula P, Lei G, Zhuang L, You MJ, Fang B, Li W, Metallo CM, Poyurovsky MV, Gan B. Nat Cell Biol. 2020 Apr;22(4):476-486. doi: 10.1038/s41556-020-0496-x. Epub 2020 Mar 30. 10.1038/s41556-020-0496-x PubMed 32231310