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pGEM mouse PEA3 [TJ#200]
(Plasmid #16346)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGEM
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    PEA3
  • Alt name
    Pea3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Entrez Gene
    Etv4 (a.k.a. RP23-434F13.1, AW414408, Pea-3, Pea3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Mouse full-length PEA3 cDNA in pGEM. To make an antisense probe, cut with XbaI or ApaI and transcribe with SP6 (ApaI cuts away the cloning artifact - see map). To make a sense probe, cut with BamHI and transcribe with T7.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM mouse PEA3 [TJ#200] was a gift from Thomas Jessell (Addgene plasmid # 16346)