pGEM mouse PEA3 [TJ#200]
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesM. musculus (mouse)
Insert Size (bp)2300
Entrez GeneEtv4 (a.k.a. RP23-434F13.1, AW414408, Pea-3, Pea3)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
Mouse full-length PEA3 cDNA in pGEM. To make an antisense probe, cut with XbaI or ApaI and transcribe with SP6 (ApaI cuts away the cloning artifact - see map). To make a sense probe, cut with BamHI and transcribe with T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEM mouse PEA3 [TJ#200] was a gift from Thomas Jessell (Addgene plasmid # 16346)
Map uploaded by the depositor.