Addgene - pGEM mouse PEA3 [TJ#200] Plasmid Data

Plasmid 16346: pGEM mouse PEA3 [TJ#200]

Gene/insert name:
PEA3
Alt name:
Pea3
Insert size:
2300
Species:
M. musculus (mouse)
Entrez Gene:
Etv4 (Pea3, Pea-3, AW414408)
Vector backbone:
pGEM
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3000
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Thomas Jessell
Terms and Licenses:
MTA

Comments:

Mouse full-length PEA3 cDNA in pGEM. To make an antisense probe, cut with XbaI or ApaI and transcribe with SP6 (ApaI cuts away the cloning artifact - see map). To make a sense probe, cut with BamHI and transcribe with T7.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16346" in your Materials and Methods section.