|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16411||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression, AAV ; adeno-associated viral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert namePTEN Neo targeting
SpeciesH. sapiens (human)
Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
This targeting vector yields 1-5% homologous recombinants in HCT116 and other cell lines. See author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Neo-PTEN was a gift from Bert Vogelstein (Addgene plasmid # 16411 ; http://n2t.net/addgene:16411 ; RRID:Addgene_16411)
For your References section:Facile methods for generating human somatic cell gene knockouts using recombinant adeno-associated viruses. Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B. Nucleic Acids Res. 2004 . 32(1):e3. 10.1093/nar/gnh009 PubMed 14704360
Map uploaded by the depositor.