pAS007
(Plasmid #164868)

• Purpose: This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.
• Depositing Lab: Keith Shearwin
• Publication: Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284.

Backbone

• Vector backbone: CRIM plasmid
• Backbone manufacturer: Haldimann and Wanner
• Backbone size w/o insert (bp): 2520
• Total vector size (bp): 2636
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: Must be maintained in a pir+ strain.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: phiKO2 operators
• Species: Synthetic
• Insert Size (bp): 116

Resource Information

• Supplemental Documents:
  • pAS0007 pLOM2-500-phiKO2.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pAS007 is a version of the pLOM2-500 loop-o-meter vector, and contains the phiKO2 cB operator sequences. pAS007 can be used as a positive control for DNA looping, in combination with pID1072, the expression plasmid for phiKO2 cB repressor.
DNA binding sites for any candidate looping proteins of interest can be used to replace the phiKO2 operators in pAS007.
pAS007 is designed to be integrated into a chromosomal lambda attachment site, using the lambda integrase helper plasmid (Addgene # 66076).