AH6114
(Bacterial strain #166038)

• Purpose: Loopometer recipient strain: lacI+ Oid+
• Depositing Lab: Keith Shearwin
• Publication: Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 6123373. doi: 10.1093/nar/gkaa1284.

Backbone

• Vector backbone: N/A
• Selectable markers: LacZ (repressed)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Spectinomycin, 100 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): This is a strain
• Growth instructions: Please grow using the following selection: Spectinomycin (20ug/ml) and Ampicillin (100ug/ml)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: N/A

Resource Information

• Supplemental Documents:
  • Strain Validation

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genotype: MG1655 rph+ ΔlacIZYA ΔλattB::lacOid_λattB_PlacUV5.lacO2_LacZ (pIT3-SH-pLacI-LacI reverse)φHK022attB

This strain contains the pInt-ts lambda helper plasmid (Addgene #66076) necessary for the genome integration of pLOM2-500 plasmid (Addgene #164868) or its derivative. This strain expresses lac repressor.