Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16496||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1700
Entrez GeneTP53I3 (a.k.a. PIG3)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBK-CMV Pig3 was a gift from Bert Vogelstein (Addgene plasmid # 16496 ; http://n2t.net/addgene:16496 ; RRID:Addgene_16496)
For your References section:A model for p53-induced apoptosis. Polyak K, Xia Y, Zweier JL, Kinzler KW, Vogelstein B. Nature. 1997 Sep 18. 389(6648):300-5. 10.1038/38525 PubMed 9305847
Map uploaded by the depositor.