pET21a.Tat(48-57)-I-SceI
(Plasmid
#165041)
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PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameI-SceI
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SpeciesS. cerevisiae (budding yeast)
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Tags
/ Fusion Proteins
- Tat(48-57) (N terminal on insert)
- 6XHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a.Tat(48-57)-I-SceI was a gift from Dokyun Na (Addgene plasmid # 165041 ; http://n2t.net/addgene:165041 ; RRID:Addgene_165041) -
For your References section:
Identification of efficient prokaryotic cell-penetrating peptides with applications in bacterial biotechnology. Lee HM, Ren J, Tran KM, Jeon BM, Park WU, Kim H, Lee KE, Oh Y, Choi M, Kim DS, Na D. Commun Biol. 2021 Feb 15;4(1):205. doi: 10.1038/s42003-021-01726-w. 10.1038/s42003-021-01726-w PubMed 33589718
