|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16527||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameSmad binding element
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
SBE-luc was made by annealing the oligonucleotides 5′-CGGAGTATGTCTAGACTGACAATG-3′ and 5′-CTAGCATTGTCAGTCTAGACATACTCCGAGCT-3′ and cloning them into the Sac1 and Nhe1 sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SBE-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16527 ; http://n2t.net/addgene:16527 ; RRID:Addgene_16527)
For your References section:Characterization of human FAST-1, a TGF beta and activin signal transducer. Zhou S, Zawel L, Lengauer C, Kinzler KW, Vogelstein B. Mol Cell. 1998 Jul . 2(1):121-7. 10.1016/S1097-2765(00)80120-3 PubMed 9702198
Map uploaded by the depositor.