|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16535||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerVogelstein Lab
- Backbone size w/o insert (bp) 4867
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Alt namePutative Tcf-4-binding sites
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
Contains 4 copies of putative Tcf-4-binding sites TRE1 (ATGTCTTTGTACTCG). The following oligonucleotide pairs were used for dimerization to construct corresponding reporters in pBV-Luc: 5'-CTAGCATGTC TTTGTACTCG ATGTCTTTGT ACTCG-3' and 5'-CTAGCGAGTA CAAAGACATC GAGTACAAAG ACATG-3' for p4XTRE1-Luc.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p4xTRE1-Luc was a gift from Bert Vogelstein (Addgene plasmid # 16535 ; http://n2t.net/addgene:16535 ; RRID:Addgene_16535)
For your References section:PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149
Map uploaded by the depositor.