Addgene - p4xTRE1-Luc Plasmid Data

Plasmid 16535: p4xTRE1-Luc

Gene/insert name:
4xTRE1
Alt name:
Putative Tcf-4-binding sites
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pBV-Luc
(Search Vector Database)
Backbone manufacturer:
Vogelstein Lab
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4867
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
SmaI
Site destroyed during cloning:
No
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA
Luciferase Limited Use Label License
Tet Systems Limited Use Label License

Comments:

Contains 4 copies of putative Tcf-4-binding sites TRE1 (ATGTCTTTGTACTCG). The following oligonucleotide pairs were used for dimerization to construct corresponding reporters in pBV-Luc: 5'-CTAGCATGTC TTTGTACTCG ATGTCTTTGT ACTCG-3' and 5'-CTAGCGAGTA CAAAGACATC GAGTACAAAG ACATG-3' for p4XTRE1-Luc.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He et al (Cell. 1999 Oct 29. 99(3):335-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16535" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

25028: pIS1-miR-335 site

16559: pGL3-OF (mutant TCF-4 sites)