Addgene - MadMyc-HA Plasmid Data

Plasmid 16557: MadMyc-HA

Gene/insert name:
MADMYC
Alt name:
c-MYC
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
Dominant negative mutant
Vector backbone:
n/a
(Search Vector Database)
Vector type:
Mammalian Expression
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Amp (50ug/uL)
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bert Vogelstein
Terms and Licenses:
MTA

Comments:

MADMYC: the amino
terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional
repression domain (aa 1-38, the Sin3-interaction region) of Mad.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of CDK4 as a target of c-MYC. Hermeking et al (Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16557" in your Materials and Methods section.