MadMyc-HA
(Plasmid #16557)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Amp (50ug/uL)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    MADMYC
  • Alt name
    c-MYC
  • Species
    H. sapiens (human)
  • Mutation
    Dominant negative mutant
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

MADMYC: the amino
terminal transactivation domain of c-Myc (aa 1-263) was removed and replaced with the amino terminal transcriptional
repression domain (aa 1-38, the Sin3-interaction region) of Mad.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MadMyc-HA was a gift from Bert Vogelstein (Addgene plasmid # 16557)
  • For your References section:

    Identification of CDK4 as a target of c-MYC. Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, Eick D, Vogelstein B, Kinzler KW. Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34. 10.1073/pnas.050586197 PubMed 10688915