|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16574||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namebeta-chorionic gonadotropin.
SpeciesH. sapiens (human)
Insert Size (bp)534
Entrez GeneCGB3 (a.k.a. CGB, CGB5, CGB7, CGB8, hCGB)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-neo-hCG was a gift from Bert Vogelstein (Addgene plasmid # 16574 ; http://n2t.net/addgene:16574 ; RRID:Addgene_16574)
For your References section:Assessing tumors in living animals through measurement of urinary beta-human chorionic gonadotropin. Shih IM, Torrance C, Sokoll LJ, Chan DW, Kinzler KW, Vogelstein B. Nat Med. 2000 Jun . 6(6):711-4. 10.1038/76299 PubMed 10835692
Map uploaded by the depositor.