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pBI-EGFP-MnSOD
(Plasmid #16612)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16612 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBI-EGFP2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MnSOD
  • Alt name
    mitochondrial gene superoxide dismutase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    704
  • Entrez Gene
    SOD2 (a.k.a. GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains full length MnSOD gene including the mitochondrial leader sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI-EGFP-MnSOD was a gift from Bert Vogelstein (Addgene plasmid # 16612 ; http://n2t.net/addgene:16612 ; RRID:Addgene_16612)
  • For your References section:

    Ferredoxin reductase affects p53-dependent, 5-fluorouracil-induced apoptosis in colorectal cancer cells. Hwang PM, Bunz F, Yu J, Rago C, Chan TA, Murphy MP, Kelso GF, Smith RA, Kinzler KW, Vogelstein B. Nat Med. 2001 Oct . 7(10):1111-7. 10.1038/nm1001-1111 PubMed 11590433