DICER KO vector
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16656||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression ; adeno-associated viral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDicer knock-out
SpeciesH. sapiens (human)
Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
The targeting construct pAAV-Neo-Dicer was made by PCR, by using bacterial artificial chromosome clone CITB 2240H23 (Invitrogen) as the template for the homology arms. A targeted insertion was made in exon 5, which is part of the helicase domain.
See Author's Map and article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DICER KO vector was a gift from Bert Vogelstein (Addgene plasmid # 16656 ; http://n2t.net/addgene:16656 ; RRID:Addgene_16656)
For your References section:The colorectal microRNAome. Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, Juhl H, Kinzler KW, Vogelstein B, Velculescu VE. Proc Natl Acad Sci U S A. 2006 Mar 7. 103(10):3687-92. 10.1073/pnas.0511155103 PubMed 16505370
Map uploaded by the depositor.