S
(Plasmid #167172)

• Purpose: Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells
• Depositing Lab: Susanne Dobler
• Publication: Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.e10. doi: 10.1016/j.cub.2021.03.089. Epub 2021 Apr 21.

Backbone

• Vector backbone: pFastBac Dual
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 5193
• Total vector size (bp): 9177
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Please note XL10-Gold was used in this study
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATP1A1-S
• Alt name: ATP1A1
• Alt name: Na+K+ATPase alpha 1
• Species: Leptodactylus latrans
• Insert Size (bp): 3069
• Promoter: PH

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: ATTCCGGATTATTCATACCGTCCCACCATCG
• 3′ sequencing primer: GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC

Gene/Insert 2

• Gene/Insert name: ATP1B1
• Alt name: Na+K+ATPase beta 1
• Species: Leptodactylus latrans
• Insert Size (bp): 915
• Promoter: P10

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: CGGGTTCCTTCCGGTATTGTCTCCTTC
• 3′ sequencing primer: ACGGACCTTTAATTCAACCCAACACAATATA

Resource Information

• Supplemental Documents:
  • pFastBac Dual with Leptodactylus ATP1A1 S _ ATP1B1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry