Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)2300
Entrez GeneSlc2a2 (a.k.a. GTT2, Glut2)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
cDNA clone isolated from a rat islet cDNA library constructed in pcDNA-1. The full length insert was excised by KpnI/XbaI and subcloned into pcDNA3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ratGLUT2 was a gift from Bernard Thorens (Addgene plasmid # 14946 ; http://n2t.net/addgene:14946 ; RRID:Addgene_14946)
For your References section:Protein kinase A-dependent phosphorylation of GLUT2 in pancreatic beta cells. Thorens B, Deriaz N, Bosco D, DeVos A, Pipeleers D, Schuit F, Meda P, Porret A. J Biol Chem. 1996 Apr 5. 271(14):8075-81. 10.1074/jbc.271.14.8075 PubMed 8626492
Map uploaded by the depositor.