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pcDNA3-myc-CUL3
(Plasmid #19893)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19893 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-myc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5512
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cullin 3
  • Alt name
    CUL3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2304
  • GenBank ID
    NM_003590
  • Entrez Gene
    CUL3 (a.k.a. CUL-3, PHA2E)
  • Tag / Fusion Protein
    • 3xmyc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc-CUL3 was a gift from Yue Xiong (Addgene plasmid # 19893 ; http://n2t.net/addgene:19893 ; RRID:Addgene_19893)
  • For your References section:

    ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407