|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19893||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5512
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecullin 3
SpeciesH. sapiens (human)
Insert Size (bp)2304
Entrez GeneCUL3 (a.k.a. CUL-3, PHA2E)
/ Fusion Protein
- 3xmyc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Y201C mutation detected in Addgene sequence. Xiong lab says that the plasmid used in the publication also had Y201C mutation and it is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc-CUL3 was a gift from Yue Xiong (Addgene plasmid # 19893 ; http://n2t.net/addgene:19893 ; RRID:Addgene_19893)
For your References section:ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407