|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19896||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5437
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namecullin 1
SpeciesH. sapiens (human)
Insert Size (bp)2260
/ Fusion Protein
- myc3 (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
When we made this plasmid, we put a Nco1 site at the start codon (original cDNA was CAATGTCG but they mutated to CcATGgCG). It made an S2A mutation. There is also a K689E mutation. These mutations have no effect on protein function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-myc3-CUL1 was a gift from Yue Xiong (Addgene plasmid # 19896 ; http://n2t.net/addgene:19896 ; RRID:Addgene_19896)
For your References section:ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Mol Cell. 1999 Apr . 3(4):535-41. 10.1016/S1097-2765(00)80482-7 PubMed 10230407