Addgene - pcDNA3-myc3-CUL4A Plasmid Data

Plasmid 19951: pcDNA3-myc3-CUL4A

Gene/insert name:
CUL4A (cullin 4A)
Alt name:
CUL4A
Insert size:
2982
Species:
H. sapiens (human)
GenBank ID:
NM_001008895
Entrez Gene:
CUL4A (RP11-391H12.1)
Fusion protein or tag:
myc3
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5437
Cloning site 5':
EcoRI
Site destroyed during cloning:
Yes
Cloning site 3':
ApaI
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers, other:
G418
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Yue Xiong
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu et al (Mol Cell. 2002 Dec . 10(6):1511-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19951" in your Materials and Methods section.