pcDNA3-myc3-CUL4A
(Plasmid #19951)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    CUL4A (cullin 4A)
  • Alt name
    CUL4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2982
  • GenBank ID
    NM_001008895
  • Entrez Gene
    CUL4A (a.k.a. RP11-391H12.1)
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site ApaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-CUL4A was a gift from Yue Xiong (Addgene plasmid # 19951)
  • For your References section:

    NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Liu J, Furukawa M, Matsumoto T, Xiong Y. Mol Cell. 2002 Dec . 10(6):1511-8. 10.1016/S1097-2765(02)00783-9 PubMed 12504025