|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16736||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namecaM kinase
SpeciesR. norvegicus (rat)
Mutationthreonine 286 mutated to aspartate
Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
Description: Constitutively active CAMKII (threonine 286 mutated to aspartate)
provided by H. Schulman (Vanderbilt) subcloned into the EcoRI site of CS2+.
Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE118 CAMKII-T286D-CS2+ was a gift from Randall Moon (Addgene plasmid # 16736 ; http://n2t.net/addgene:16736 ; RRID:Addgene_16736)