XE120 CAMKII-K42M 1-271-CS2+
(Plasmid
#16734)
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Depositing Lab
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Publication
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Sequence Information
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Depositor Sequences: None.
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Addgene Sequences: Partial (1)
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 16734 | Plasmid sent as bacteria in agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCAMKII-K42M 1-271
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Alt nameCAMKII
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Alt namecaM kinase
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SpeciesR. norvegicus (rat)
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MutationC-terminal truncation of K42M kinase
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Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (unknown if destroyed)
- 3′ cloning site EcoR1 (unknown if destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
Depositor Comments
Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE120 CAMKII-K42M 1-271-CS2+ was a gift from Randall Moon (Addgene plasmid # 16734)