XE120 CAMKII-K42M 1-271-CS2+
(Plasmid #16734)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)
• Sequence Information:

  • Depositor Sequences: None.
  • Addgene Sequences: Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	16734	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

Available to Academic and Nonprofits Only

Backbone

• Vector backbone: pCS2+
  (Search Vector Database)
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAMKII-K42M 1-271
• Alt name: CAMKII
• Alt name: caM kinase
• Species: R. norvegicus (rat)
• Mutation: C-terminal truncation of K42M kinase
• Entrez Gene: Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Terms and Licenses:
  • UBMTA

Depositor Comments

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.