Addgene - XE120 CAMKII-K42M 1-271-CS2+ Plasmid Data

Plasmid 16734: XE120 CAMKII-K42M 1-271-CS2+

Gene/insert name:
CAMKII-K42M 1-271
Alt name:
CAMKII
Alt name:
caM kinase
Species:
R. norvegicus (rat)
Entrez Gene:
Camk2a (PK2CDD, PKCCD)
Mutation:
C-terminal truncation of K42M kinase
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
Cloning site 5':
EcoR1
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoR1
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
UBMTA

Comments:

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16734" in your Materials and Methods section.