XE120 CAMKII-K42M 1-271-CS2+
(Plasmid #16734)

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Depositing Lab

Randall Moon
Publication

Xenopus Expression Plasmids (unpublished)
Price

$65 (USD)

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Available to Academic and Nonprofits Only

Backbone

Vector backbone
pCS2+
(Search Vector Database)
Backbone size w/o insert (bp) 4095
Vector type
Xenopus Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Sequence Information

Depositor Sequences

None.
Addgene Sequences

Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

Gene/Insert name
CAMKII-K42M 1-271
Alt name
CAMKII
Alt name
caM kinase
Species
R. norvegicus (rat)
Mutation
C-terminal truncation of K42M kinase
Entrez Gene
Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site EcoR1 (unknown if destroyed)
3′ cloning site EcoR1 (unknown if destroyed)
5′ sequencing primer SP6

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA

Depositor Comments

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

XE120 CAMKII-K42M 1-271-CS2+ was a gift from Randall Moon (Addgene plasmid # 16734)

Map generated by Addgene from plasmid data.

XE120 CAMKII-K42M 1-271-CS2+ (Plasmid #16734)