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XE120 CAMKII-K42M 1-271-CS2+
(Plasmid #16734)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    CAMKII-K42M 1-271
  • Alt name
    CAMKII
  • Alt name
    caM kinase
  • Species
    R. norvegicus (rat)
  • Mutation
    C-terminal truncation of K42M kinase
  • Entrez Gene
    Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE120 CAMKII-K42M 1-271-CS2+ was a gift from Randall Moon (Addgene plasmid # 16734)