Addgene - XE120 CAMKII-K42M 1-271-CS2+ Plasmid Data

Plasmid 16734: XE120 CAMKII-K42M 1-271-CS2+

Gene/insert name:
CAMKII-K42M 1-271
Alt name:
CAMKII
Alt name:
caM kinase
Species:
R. norvegicus (rat)
Entrez Gene:
Camk2a (PK2CDD, PKCCD)
Mutation:
C-terminal truncation of K42M kinase
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Xenopus Expression
Backbone size w/o insert (bp):
4095
Cloning site 5':
EcoR1
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoR1
Site destroyed during cloning:
Unknown
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Randall Moon
Terms and Licenses:
UBMTA

Comments:

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16734" in your Materials and Methods section.