Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10816||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePKD2 RNAi
SpeciesH. sapiens (human)
Insert Size (bp)64
Entrez GenePRKD2 (a.k.a. HSPC187, PKD2, nPKC-D2)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCACA
TGACCCCACG TCGGCCTTCA AGAGAGGCCG ACGTGGGGTC ATGTTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAACATGA CCCCACGTCG GCCTCTCTTG AAGGCCGACG TGGGGTCATG TGGG-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSUPER.PKD2.RNAi was a gift from Alex Toker (Addgene plasmid # 10816 ; http://n2t.net/addgene:10816 ; RRID:Addgene_10816)
For your References section:Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Storz P, Doppler H, Toker A. Mol Cell Biol. 2004 Apr . 24(7):2614-26. 10.1128/MCB.24.7.2614-2626.2004 PubMed 15024053