LES2A-CRE
(Plasmid #167933)

• Purpose: Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.
• Depositing Lab: Ronald Germain
• Publication: Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 10.4049/immunohorizons.2000082.

Backbone

• Vector backbone: lentiCas9-Blast
• Backbone manufacturer: Feng Zhang (Addgene Plasmid #52962)
• Total vector size (bp): 10009
• Modifications to backbone: Replacing the XbaI-EcoRI fragment in the backbone with the mScarlet-P2A-CRE fragment.
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet-P2A-CRE
• Insert Size (bp): 1790
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: 5'-gggacagcagagatccagtt-3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry