XE69 Xenopus Beta-catenin in GFP
(Plasmid #16839)

• Depositing Lab: Randall Moon
• Publication: Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4100
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta-catenin GFP
• Alt name: beta catenin
• Species: X. laevis (frog)
• Insert Size (bp): 2300
• Entrez Gene: ctnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (unknown if destroyed)
• 3′ cloning site: Cla1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

bGFP of Hiem et al., in Cla I site, with stop codon at 3' end. No myc epitope.
Linearize with Not 1 and transcribe with SP6.

Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.