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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 16839 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-catenin GFP
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Alt namebeta catenin
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SpeciesX. laevis (frog)
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Insert Size (bp)2300
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Entrez Genectnnb1.L (a.k.a. XELAEV_18031149mg, B-catenin, beta-catenin, ctnnb, ctnnb1, ctnnb1-a, ctnnb1-b)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (unknown if destroyed)
- 3′ cloning site Cla1 (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
Depositor Comments
bGFP of Hiem et al., in Cla I site, with stop codon at 3' end. No myc epitope.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE69 Xenopus Beta-catenin in GFP was a gift from Randall Moon (Addgene plasmid # 16839 ; http://n2t.net/addgene:16839 ; RRID:Addgene_16839)
Map uploaded by the depositor.
