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ZP61 Isthmin
(Plasmid #16881)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16881 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    isthmin
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    ism1

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Zebrafish Isthmin cDNA, clone 27j15 from the microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library) in pSPORT1. A clone that is slightly longer at the 5' end was identified in the 2nd array round, so this is not exactly full-length. For antisense probe synthesis.
Expressed on dorsal side early, in a dorsal high to ventral low gradient in margin, during gastrulation around axial mesoderm, later strongly at MHB. For antisense: Asp718, SP6.

Citation: G. Weidinger and R. T. Moon (2005). Development.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP61 Isthmin was a gift from Randall Moon (Addgene plasmid # 16881 ; http://n2t.net/addgene:16881 ; RRID:Addgene_16881)