U6-U57C
(Plasmid
#169928)
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PurposeWT U6 with a U57C mutation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS314
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4783
- Total vector size (bp) 1217
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameU6 U57C
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Alt namepRS314-U6 U57C
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Alt namepAAH1028
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SpeciesS. cerevisiae (budding yeast)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
U6-U57C was a gift from Aaron Hoskins (Addgene plasmid # 169928 ; http://n2t.net/addgene:169928 ; RRID:Addgene_169928) -
For your References section:
Saccharomyces cerevisiae Ecm2 Modulates the Catalytic Steps of pre-mRNA Splicing. van der Feltz C, Nikolai B, Schneider C, Paulson JC, Fu X, Hoskins AA. RNA. 2021 Feb 5. pii: rna.077727.120. doi: 10.1261/rna.077727.120. 10.1261/rna.077727.120 PubMed 33547186
