pUK21
(Plasmid #49788)

• Purpose: (Empty Backbone) E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)
• Depositing Lab: Joachim Messing
• Publication: Vieira et al Gene. 1991 Apr;100:189-94.

Backbone

• Vector backbone: pUC21 (Addgene plasmid #49787)
• Backbone manufacturer: Messing Lab
• Backbone size (bp): 3090
• Modifications to backbone: The bla gene of pUC21 was replaced with the Kanamycin resistance gene from Tn903 by cutting pUC21 with BspHI, filling in the ends, and inserting the Tn903 fragment. The HindIII and SmaI sites have been removed from the Kanamycin Resistance gene so that these sites within the MCS are now unique and can be used for cloning.
• Vector type: cloning vector
• Promoter: lacZ

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • Genbank
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry