Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pOK12
(Plasmid #49789)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49789 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC21 (Addgene plasmid #49787)
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 2135
  • Modifications to backbone
    The lac region and MCS are identical to pUC21, The KmR gene is from Tn905 and the ori is P15A from pACYC 177.
  • Vector type
    cloning vector
  • Promoter lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOK12 was a gift from Joachim Messing (Addgene plasmid # 49789 ; http://n2t.net/addgene:49789 ; RRID:Addgene_49789)
  • For your References section:

    New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257