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pETDuet-1 TIM9,10
(Plasmid #170280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 170280 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag)
  • Alt name
    Tim9
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TIM9 (a.k.a. YEL020W-A)
  • Entrez Gene
    TIM10 (a.k.a. YHR005C-A, MRS11)
  • Promoter Tim9: via NdeI/XhoI into the MCS2; Tim10: via BamHI/HindIII into the MCS1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-1 TIM9,10 was a gift from Paul Schanda (Addgene plasmid # 170280 ; http://n2t.net/addgene:170280 ; RRID:Addgene_170280)
  • For your References section:

    Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Weinhaupl K, Lindau C, Hessel A, Wang Y, Schutze C, Jores T, Melchionda L, Schonfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P. Cell. 2018 Nov 15;175(5):1365-1379.e25. doi: 10.1016/j.cell.2018.10.039. 10.1016/j.cell.2018.10.039 PubMed 30445040