pETDuet-1 TIM9,10
(Plasmid #170280)

• Purpose: Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
• Depositing Lab: Paul Schanda
• Publication: Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25. doi: 10.1016/j.cell.2018.10.039.

Backbone

• Vector backbone: pETDuet-1
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 5900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag)
• Alt name: Tim9
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: TIM9 (a.k.a. YEL020W-A)
• Entrez Gene: TIM10 (a.k.a. YHR005C-A, MRS11)
• Promoter: Tim9: via NdeI/XhoI into the MCS2; Tim10: via BamHI/HindIII into the MCS1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry