Tol2-kdrl:mCherry-caax
(Plasmid
#194287)
-
PurposeExpression plasmid for zebrafish or Danionella cerebrum
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepDestTol2pA2 from the Tol2Kit (Kwan et al., 2007)
-
Vector typeZebrafish or Danionella cerebrum Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namekdrl promoter from Addgene plasmid # 78687
-
SpeciesD. rerio (zebrafish)
-
Entrez Genekdrl (a.k.a. flk, flk-1, flk1, kdr, kdra, vegfr-2, vegfr2, vegfr2a, vegfr4, vegr2, wu:fk52c05)
- Promoter kdrl(flk1)
-
Tag
/ Fusion Protein
- mCherryCAAX from the Tol2kit (Kwan et al., 2007)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Tol2-kdrl:mCherry-caax was a gift from Pui-Ying Lam (Addgene plasmid # 194287 ; http://n2t.net/addgene:194287 ; RRID:Addgene_194287) -
For your References section:
Longitudinal in vivo imaging of adult Danionella cerebrum using standard confocal microscopy. Lam PY. Dis Model Mech. 2022 Dec 1;15(12):dmm049753. doi: 10.1242/dmm.049753. Epub 2022 Dec 22. 10.1242/dmm.049753 PubMed 36398624
