Epac-S-H170
(Plasmid #170352)

• Purpose: mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
• Depositing Lab: Kees Jalink
• Publication: Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. doi: 10.1371/journal.pone.0122513. eCollection 2015.

Backbone

• Vector backbone: pAdeno
• Backbone size w/o insert (bp): 36000
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST)
• Species: H. sapiens (human)
• Mutation: Deletion of DEP domain, Catalytically dead T781A & F782A, increased affinity for cAMP Q270E
• Entrez Gene: RAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • H170_pAd-mT2-EPACdDEPCD_cp173V(ST)_V(ST).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry