pHCMM4
(Plasmid #170359)

• Purpose: Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter
• Depositing Labs: Hugo Cesar Medina-Munoz, Marvin Wickens
• Publication: Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 10.1073/pnas.1921408117. Epub 2020 Sep 9.

Backbone

• Vector backbone: NA
• Backbone size w/o insert (bp): 4872
• Total vector size (bp): 9240
• Vector type: Yeast Expression
• Selectable markers: LEU2, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PCYC1_SEC61_mCherry_LEU2_TCYC1
• Species: C. elegans (nematode), S. cerevisiae (budding yeast)
• Insert Size (bp): 4368
• Promoter: PCYC1

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry