pDONR207-MUM2sp-Citrine-ADPG2
(Plasmid #170725)

• Purpose: Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
• Depositing Lab: George Haughn
• Publication: McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077.

Backbone

• Vector backbone: pDONR207
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5585
• Total vector size (bp): 5474
• Vector type: Gateway Donor vector / entry clone

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For selection use 7 ug/ml Gentamicin
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2
• Alt name: ADPG2
• Alt name: At2g41850
• Alt name: Q8RY29 (Uniprot)
• Species: A. thaliana (mustard weed)
• GenBank ID: AC002339
• Entrez Gene: PGAZAT (a.k.a. AT2G41850, ADPG2, ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2, T11A7.5, T11A7_5, polygalacturonase abscission zone A. thaliana)
• Tag / Fusion Protein:
  • Citrine tag (714bp) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: AatII (not destroyed)
• 5′ sequencing primer: TAACGCTAGCATGGATCTC
• 3′ sequencing primer: GTAACATCAGAGATTTTGAGACA

Resource Information

• Supplemental Documents:
  • pDONR207-MUM2sp-Citrine-ADPG2 - Snapgene Plasmid Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).