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Pectin Modification in Seed Coat Mucilage by In Vivo Expression of Rhamnogalacturonan-I- and Homogalacturonan-Degrading Enzymes.
McGee R, Dean GH, Wu D, Zhang Y, Mansfield SD, Haughn GW
Plant Cell Physiol. 2021 Jun 1. pii: 6290394. doi: 10.1093/pcp/pcab077. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170720pDONR207-MUM2sp-RglAGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)
170721pDONR207-MUM2sp-RglBGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)
170722pDONR207-MUM2sp-PelAGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)
170723pDONR207-MUM2sp-ADPG2Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
170725pDONR207-MUM2sp-Citrine-ADPG2Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Antibodies from Article