-1.5 kb goosecoid luciferase
(Plasmid #17159)

• Depositing Lab: Ken Cho
• Publication: Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Backbone

• Vector backbone: pOLuc
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: goosecoid promoter
• Alt name: goosecoid
• Species: X. laevis (frog)
• Insert Size (bp): 1500
• Entrez Gene: gsc-a (a.k.a. Xgsc, goosecoid)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV / SmaI (destroyed during cloning)
• 3′ cloning site: PstI / SmaI (destroyed during cloning)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987).