-1.5 kb goosecoid luciferase
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17159||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert namegoosecoid promoter
SpeciesX. laevis (frog)
Insert Size (bp)1500
Entrez Genegsc-a (a.k.a. Xgsc, goosecoid)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV / SmaI (destroyed during cloning)
- 3′ cloning site PstI / SmaI (destroyed during cloning)
- 5′ sequencing primer SP6
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
The - 1500gsc/Luc reporter was constructed from a blunt-ended EcoRV-PstI 1.5kb Xenopus gsc gene fragment carrying 3 bp of exon 1 and 5'-promoter sequences inserted into the SmaI site of the promoterless luciferase vector pOLuc (de Wet et al. 1987).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:-1.5 kb goosecoid luciferase was a gift from Ken Cho (Addgene plasmid # 17159 ; http://n2t.net/addgene:17159 ; RRID:Addgene_17159)
For your References section:Molecular mechanisms of Spemann's organizer formation: conserved growth factor synergy between Xenopus and mouse. Watabe T, Kim S, Candia A, Rothbacher U, Hashimoto C, Inoue K, Cho KW. Genes Dev. 1995 Dec 15. 9(24):3038-50. 10.1101/gad.9.24.3038 PubMed 8543150