pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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GenBank

SnapGene

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> Addgene NGS Result TCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGG ATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCG GTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTG GACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCA GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCC CGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTT CCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGC CTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC GCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAG CGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAATGGTGCACT CTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGT CATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCC GCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAC GCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATC CGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGG GCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATAC CGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTG TGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGC TTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAA TGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGT TGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGC TAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTG GCCAGGACCCAACGCTGCCCGAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTT CTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTG AATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAA CGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAG GCGGCATAAATCGCCGTGACGATCAGCGGTCCAATGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATC CTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGAT GCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACGCCAGCAAGACG TAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGAC CAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATG ATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGT TGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTT GCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCG GGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA AATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTAC CGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCG TTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACA TGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAAT GCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCT GGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTC ATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAG GCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTG TTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTT TCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGG CATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCG CTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGT GCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAG CGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAAC CGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGA AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCG CGGCAGCCATATGGCTAGCTGGAGCCATCCGCAGTTTGAAAAAGGTGGTGGTAGCGGTGGTGGTTCAGGT GGTAGTGCATGGTCACACCCTCAGTTTGAGAAAATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAG AGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTT CTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAA ATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATCCAAGCTGATCAGACCCCTGAAGATTTGGACA TGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCATGACCACTACCATGAAGAT CAGCATCGAGTTCTTGGAGCCCTTCCGCATGACGAAGTGGCAGGAGTCCACGCGCCGCAATAAGAACAAT AAAGAGTTTGTGCGTGGCCAAGCGTTTGCCCGTTGGCACCGCAACAAGAAAGATAACACCAAGGGTCGTC CGTACATTACTGGTACGCTCCTGCGCTCAGCGGTGATTCGCTCCGCGGAGAACCTGCTGACACTGAGTGA CGGTAAGATCTCCGAGAAAACCTGCTGCCCGGGGAAATTCGACACGGAAGATAAGGACCGTTTACTCCAG CTGCGTCAACGCTCCACGCTGCGTTGGACAGACAAGAATCCGTGTCCCGACAATGCGGAAACGTATTGTC CGTTCTGTGAGCTCCTCGGCCGCTCCGGAAATGACGGTAAGAAAGCAGAAAAGAAGGACTGGCGCTTCCG CATCCATTTTGGTAACCTTTCCCTGCCGGGCAAGCCCGACTTCGACGGTCCGAAGGCAATTGGTTCACAG CGCGTATTAAATCGTGTGGATTTTAAGTCGGGAAAGGCACATGACTTCTTCAAGGCTTATGAGGTGGACC ATACCCGCTTCCCTCGCTTTGAAGGGGAGATCACCATCGACAATAAAGTGTCCGCCGAGGCACGTAAGTT GCTGTGTGACAGCCTTAAATTCACGGATCGCCTGTGTGGAGCCCTGTGCGTCATCCGCTTCGACGAGTAT ACACCGGCCGCGGACAGCGGTAAGCAAACCGAGAATGTGCAGGCAGAGCCCAACGCAAACCTGGCGGAGA AAACTGCCGAGCAAATTATCTCGATCCTCGATGACAACAAGAAAACGGAATACACCCGCCTTTTAGCGGA CGCCATCCGCTCATTGCGTCGCTCCTCAAAACTTGTTGCAGGTTTGCCCAAGGATCACGATGGTAAGGAC GATCATTACTTGTGGGACATTGGGAAGAAGAAGAAGGATGAGAATTCGGTTACCATCCGTCAGATTCTTA CCACGAGTGCCGACACGAAGGAGCTGAAGAACGCTGGTAAGTGGCGCGAGTTTTGTGAGAAGCTTGGTGA GGCGCTTTATTTGAAGAGTAAGGATATGAGCGGCGGGTTGAAGATTACACGCCGTATTCTCGGGGACGCA GAGTTTCATGGCAAGCCAGATCGCTTGGAAAAGTCCCGTAGTGTCTCGATCGGAAGCGTGTTAAAGGAAA CCGTGGTGTGCGGAGAACTCGTCGCAAAGACCCCGTTCTTCTTTGGCGCGATCGACGAGGATGCAAAACA AACTGACTTACAGGTACTGCTTACTCCGGACAATAAGTATCGCCTGCCGCGCAGCGCCGTCCGCGGCATC CTTCGCCGCGATCTTCAAACGTACTTTGACAGTCCCTGTAATGCGGAGTTAGGCGGTCGTCCATGTATGT GCAAGACCTGTCGTATCATGCGTGGCATTACGGTTATGGACGCGCGCTCCGAGTATAACGCGCCCCCGGA GATTCGTCACCGTACCCGCATTAATCCGTTTACGGGAACAGTTGCTGAGGGCGCCCTGTTTAATATGGAA GTGGCCCCGGAAGGGATCGTATTCCCATTCCAATTACGCTATCGCGGCTCTGAGGACGGGCTCCCAGACG CGCTCAAGACCGTGCTGAAGTGGTGGGCAGAGGGGCAAGCTTTTATGTCAGGGGCCGCCAGTACCGGGAA AGGCCGTTTTCGTATGGAGAATGCCAAATATGAAACCTTGGATCTGAGCGATGAGAATCAACGCAATGAC TACTTGAAGAATTGGGGCTGGCGTGACGAGAAGGGTCTTGAGGAGCTTAAGAAACGTTTAAATTCAGGTC TGCCGGAGCCTGGCAATTACCGTGACCCGAAGTGGCATGAAATCAACGTGTCAATCGAGATGGCGAGTCC CTTCATCAACGGCGACCCTATCCGCGCAGCTGTAGACAAGCGTGGGACGGACGTCGTGACCTTCGTTAAG TACAAGGCGGAAGGGGAAGAAGCAAAGCCCGTGTGCGCATATAAGGCGGAGTCGTTTCGCGGCGTCATTC GTTCGGCTGTAGCACGCATTCACATGGAAGATGGAGTGCCCCTGACTGAGCTTACTCACTCAGATTGCGA GTGTTTGCTCTGTCAAATCTTCGGGTCGGAATATGAGGCTGGTAAGATTCGTTTCGAAGATTTAGTATTT GAGAGTGACCCGGAGCCGGTCACCTTCGATCACGTTGCAATCGATCGCTTCACCGGCGGTGCCGCTGACA AGAAGAAGTTCGACGACAGTCCTTTGCCAGGGTCGCCGGCGCGCCCTCTGATGCTGAAGGGTTCCTTCTG GATTCGCCGTGACGTTCTCGAAGATGAGGAGTATTGCAAAGCGCTCGGGAAAGCATTAGCCGATGTAAAT AATGGTTTATATCCTCTGGGTGGAAAATCCGCCATCGGATACGGACAAGTTAAGAGCCTGGGTATCAAGG GCGACGATAAGCGTATCAGCCGTTTAATGAATCCGGCCTTCGACGAAACCGACGTGGCGGTTCCAGAGAA GCCTAAAACCGATGCCGAGGTCCGTATCGAAGCTGAGAAGGTATATTATCCCCACTACTTTGTGGAACCG CACAAGAAGGTAGAGCGCGAGGAGAAGCCGTGCGGTCATCAAAAGTTTCATGAGGGACGCCTTACCGGTA AGATTCGTTGTAAACTTATTACGAAAACGCCTCTCATTGTGCCGGACACCAGCAATGATGACTTCTTTCG CCCTGCGGACAAAGAGGCCCGCAAGGAGAAGGACGAGTATCACAAATCCTATGCCTTCTTTCGCCTGCAT AAGCAAATCATGATCCCCGGTAGTGAACTCCGCGGGATGGTGTCCTCTGTTTACGAAACGGTTACAAATT CATGTTTCCGTATCTTTGACGAGACTAAGCGTCTTAGTTGGCGCATGGATGCCGATCACCAAAACGTTTT ACAAGATTTCTTACCGGGCCGTGTTACGGCCGACGGCAAGCACATTCAAAAGTTTTCCGAGACTGCACGT GTACCGTTTTATGACAAAACGCAAAAGCACTTTGACATTCTCGACGAGCAGGAAATTGCCGGTGAAAAGC CGGTCCGCATGTGGGTGAAGCGTTTCATTAAGCGCCTGAGCCTGGTGGACCCTGCAAAGCACCCACAAAA GAAGCAAGACAATAAGTGGAAACGCCGCAAGGAAGGTATTGCGACGTTCATTGAGCAGAAGAATGGGTCT TACTATTTTAACGTGGTCACCAACAATGGCTGCACCAGCTTTCACTTGTGGCACAAGCCAGACAACTTCG ACCAAGAGAAGTTAGAGGGCATCCAAAACGGGGAGAAGTTAGATTGCTGGGTTCGTGATAGCCGTTACCA AAAGGCATTTCAAGAAATCCCGGAGAACGATCCGGACGGATGGGAGTGTAAGGAAGGTTATCTGCATGTG GTGGGCCCTTCGAAGGTTGAATTTAGCGACAAGAAGGGCGATGTCATTAATAACTTCCAAGGGACTCTCC CATCAGTGCCCAATGATTGGAAAACGATTCGTACAAACGACTTTAAGAACCGCAAACGTAAGAACGAGCC CGTTTTCTGTTGCGAGGATGACAAGGGGAACTACTACACGATGGCCAAATATTGTGAAACGTTCTTCTTC GACCTGAAAGAGAACGAGGAGTATGAGATCCCCGAGAAGGCACGTATCAAGTACAAGGAGTTATTACGCG TGTACAACAACAATCCGCAAGCGGTTCCCGAGAGCGTGTTCCAGAGTCGCGTAGCGCGCGAGAACGTAGA AAAGCTGAAGTCTGGGGATTTGGTGTATTTCAAGCACAACGAGAAATATGTTGAGGACATCGTACCGGTT CGTATTTCACGTACCGTAGACGACCGCATGATCGGAAAGCGTATGTCGGCTGATTTGCGTCCATGTCACG GTGACTGGGTAGAGGACGGGGACCTCTCTGCGTTGAATGCATATCCAGAGAAGCGCCTTTTATTGCGTCA CCCTAAGGGCCTTTGCCCGGCATGTCGCCTTTTCGGAACGGGTAGCTACAAGGGCCGCGTTCGTTTTGGC TTCGCGAGCCTGGAGAACGACCCGGAGTGGTTGATTCCTGGAAAGAATCCCGGTGATCCATTCCACGGCG GGCCAGTGATGCTGAGCTTGCTGGAGCGCCCGCGTCCCACCTGGAGCATTCCTGGCAGTGATAATAAGTT CAAAGTTCCAGGCCGTAAGTTCTACGTTCACCATCATGCTTGGAAAACGATTAAGGACGGTAACCATCCG ACCACCGGCAAAGCCATTGAACAATCGCCAAATAACCGCACGGTTGAAGCGCTCGCCGGTGGCAACAGCT TCAGCTTCGAAATTGCGTTCGAAAATTTGAAGGAGTGGGAGTTGGGCCTGCTGATCCATAGCCTGCAGCT TGAAAAGGGCTTGGCCCATAAGCTCGGTATGGCTAAGAGCATGGGCTTTGGCTCAGTAGAGATCGACGTT GAGAGTGTCCGCCTGCGTAAAGATTGGAAGCAGTGGCGCAATGGTAATTCAGAGATCCCAAATTGGTTGG GCAAGGGGTTTGCAAAGCTGAAGGAGTGGTTCCGCGACGAGCTGGACTTCATCGAGAATTTGAAGAAATT ACTGTGGTTCCCCGAAGGTGATCAAGCTCCGCGCGTATGTTACCCCATGCTTCGCAAGAAGGATGACCCT AATGGAAACTCAGGTTATGAGGAGTTGAAAGACGGCGAATTTAAGAAGGAAGATCGTCAGAAGAAGCTTA CAACACCATGGACGCCGTGGGCTTGAGCGGCCGCACTCGAGGCCCGAAAGGAAGCTGAGTTGGCTGCTGC CACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA GGAGGAACTATATCCGGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCAAGCCTATGCCTACAGC ATCCAGGGTGACGGTGCCGAGGATGACGATGAGCGCATTGTTAGATTTCATACACGGTGCCTGACTGCGT TAGCAATTTAACTGTGATAAACTACCGCATTAAAGCTTATCGATGATAAGCTGTCAAACATGAGAATTCT TGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGA CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAA TATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG AAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCT CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT CTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATT CTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGA ATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCG CAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCG GTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG ATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT AATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATC

pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon Sequences (1)

Full Sequences from Addgene (1)

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