|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17254||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2641
Growth in Bacteria
Gene/Insert namepie-1 3'utr in pDONRP2RP3
SpeciesC. elegans (nematode)
Insert Size (bp)122
- Cloning method Gateway Cloning
- 5′ cloning site attP2R (destroyed during cloning)
- 3′ cloning site attP3 (destroyed during cloning)
- 5′ sequencing primer M13Forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM5.47 was a gift from Geraldine Seydoux (Addgene plasmid # 17254 ; http://n2t.net/addgene:17254 ; RRID:Addgene_17254)