pCG150
(Plasmid #17247)

• Depositing Lab: Geraldine Seydoux
• Publication: Seydoux Lab - Third Generation Vector Kit (unpublished)

Backbone

• Vector backbone: pDESTR4R3
• Backbone size w/o insert (bp): 4115
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: Needs ccdB resistant strain.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: unc-119 rescue fragment in pDESTR4R3
• Species: C. elegans (nematode)
• Insert Size (bp): 2237

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR4 (destroyed during cloning)
• 3′ cloning site: attR3 (destroyed during cloning)
• 5′ sequencing primer: M13Reverse

Resource Information

• Supplemental Documents:
  • Cloning protocol
  • Bombardment protocol
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry