pcDNA3.3-NL63-D14
(Plasmid #172666)

• Purpose: encodes NL63 spike protein, for pseudovirus production
• Depositing Lab: David Nemazee
• Publication: Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.

Backbone

• Vector backbone: pcDNA3.3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 10000
• Vector type: Mammalian Expression, Lentiviral, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HCNV63gp2
• Alt name: human NL63 coronavirus Spike protein
• Species: human coronavirus NL63
• Insert Size (bp): 4000
• Mutation: c-terminal deletion of 14aa
• GenBank ID: 2943499
• Entrez Gene: HCNV63gp2 (a.k.a. HCNV63gp2)
• Promoter: CMV
• Tag / Fusion Protein:
  • NA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.04.11.487879v1.