TurboID-mNeongreen-3xFLAG fusion pos3
(Plasmid
#172864)
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PurposeC. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR™ P2r-P3
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsThis plasmid is provided in pre-transformed ccdB survival cells. Other common strains like DH5 alpha or NEB stable cells can also be used.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTurboID
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Insert Size (bp)2199
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Tags
/ Fusion Proteins
- myc (C terminal on insert)
- mNeongreen (C terminal on insert)
- FLAG (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.01.438103v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TurboID-mNeongreen-3xFLAG fusion pos3 was a gift from Mario de Bono (Addgene plasmid # 172864 ; http://n2t.net/addgene:172864 ; RRID:Addgene_172864) -
For your References section:
Interactome analysis of Caenorhabditis elegans synapses by TurboID-based proximity labeling. Artan M, Barratt S, Flynn SM, Begum F, Skehel M, Nicolas A, de Bono M. J Biol Chem. 2021 Sep;297(3):101094. doi: 10.1016/j.jbc.2021.101094. Epub 2021 Aug 18. 10.1016/j.jbc.2021.101094 PubMed 34416233
Map uploaded by the depositor.
