ChrII_ttTi5605_loxP_myo2_neoR
(Plasmid #194056)

• Purpose: Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome
• Depositing Lab: Kota Mizumoto
• Publication: Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. doi: 10.1016/j.xpro.2022.102028.

Backbone

• Vector backbone: loxP_myo2_neoR
• Backbone manufacturer: John Calarco's lab at University of Toronto
• Modifications to backbone: 920 bp and 967 bp 5' and 3' homology arm sequences pf ttTi5605 locus are cloned at SacII and NotI sites
• Vector type: Worm Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ttTi5605
• Species: C. elegans (nematode)
• Promoter: None

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13(-41) Forward
• 3′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • ChrII_ttTi5605_loxP_myo2_neoR.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry