Skip to main content
Addgene

pFA6a-mCherry-sfGFP-kanMX (pMaM17)
(Plasmid #173452)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 173452 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFA6a-kanMX
  • Backbone size w/o insert (bp) 3938
  • Total vector size (bp) 5614
  • Modifications to backbone
    Backbone was linearized before the KanMx marker and mCherry-sfGFP-ADH1ter was introduced.
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mCherry-sfGFP-ADH1ter
  • Insert Size (bp)
    1703
  • Promoter no promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer agagtgcaccatatggac
  • 3′ sequencing primer cacatcatgcccctgagc
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was constructed and published in Khmelinskii et al. 2012 Nat Biotechnol https://www.ncbi.nlm.nih.gov/pubmed/22729030. But it was not deposited in Addgene before.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Khmelinskii et al. 2012 Nat Biotechnol https://www.ncbi.nlm.nih.gov/pubmed/22729030

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-mCherry-sfGFP-kanMX (pMaM17) was a gift from Anton Khmelinskii (Addgene plasmid # 173452 ; http://n2t.net/addgene:173452 ; RRID:Addgene_173452)
  • For your References section:

    Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Khmelinskii A, Keller PJ, Bartosik A, Meurer M, Barry JD, Mardin BR, Kaufmann A, Trautmann S, Wachsmuth M, Pereira G, Huber W, Schiebel E, Knop M. Nat Biotechnol. 2012 Jun 24;30(7):708-14. doi: 10.1038/nbt.2281. 10.1038/nbt.2281 PubMed 22729030