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pPSU1
(Plasmid #89439)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89439 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC9
  • Backbone size w/o insert (bp) 2635
  • Total vector size (bp) 10000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    500 bp EcoRV fragment
  • Alt name
    lambda DNA position 650-1149
  • Species
    phage lambda
  • Insert Size (bp)
    500
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HIndIII (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    1000 bp EcoRV fragment
  • Alt name
    lambda DNA position 6681-7681
  • Species
    phage lambda
  • Insert Size (bp)
    1000
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    1500 bp EcoRV fragment
  • Alt name
    lambda DNA position 28223-29710
  • Species
    phage lambda
  • Insert Size (bp)
    1500
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    2000 bp EcoRV fragment
  • Alt name
    lambda DNA position 36060-37994
  • Species
    phage lambda
  • Insert Size (bp)
    2000
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    500 bp PstI fragment
  • Alt name
    lambda DNA position 28223-28663
  • Species
    phage lambda
  • Insert Size (bp)
    500
  • GenBank ID
    NC_001416.1

Gene/Insert 6

  • Gene/Insert name
    700 bp PstI fragment
  • Alt name
    lambda DNA position 8783-9468
  • Species
    phage lambda
  • Insert Size (bp)
    700
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 6

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 7

  • Gene/Insert name
    800 bp PstI fragment
  • Alt name
    G9a position 2757-3535
  • Alt name
    EHMT2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    800
  • GenBank ID
    NM_006709.4

Cloning Information for Gene/Insert 7

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 8

  • Gene/Insert name
    900 bp PstI fragment
  • Alt name
    lambda DNA position 143381-15280
  • Species
    phage lambda
  • Insert Size (bp)
    900
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 8

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 9

  • Gene/Insert name
    1000 bp PstI fragment
  • Alt name
    Bmi1 position 5-979
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • GenBank ID
    NM_005180.8

Gene/Insert 10

  • Gene/Insert name
    2000 bp PstI fragment
  • Alt name
    lambda DNA position 28670-29710 and 36060-37994
  • Species
    phage lambda
  • Insert Size (bp)
    2000
  • GenBank ID
    NC_001416.1

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSU1 was a gift from Song Tan (Addgene plasmid # 89439)
  • For your References section:

    The pPSU Plasmids for Generating DNA Molecular Weight Markers. Henrici RC, Pecen TJ, Johnston JL, Tan S. Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. 10.1038/s41598-017-02693-1 [pii] PubMed 28550309