pPSU1
(Plasmid #89439)

• Purpose: High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
• Depositing Lab: Song Tan
• Publication: Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.

Backbone

• Vector backbone: pUC9
• Backbone size w/o insert (bp): 2635
• Total vector size (bp): 10000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: 500 bp EcoRV fragment
• Alt name: lambda DNA position 650-1149
• Species: phage lambda
• Insert Size (bp): 500
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HIndIII (not destroyed)
• 3′ cloning site: None (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: 1000 bp EcoRV fragment
• Alt name: lambda DNA position 6681-7681
• Species: phage lambda
• Insert Size (bp): 1000
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (not destroyed)
• 3′ cloning site: None (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 3

• Gene/Insert name: 1500 bp EcoRV fragment
• Alt name: lambda DNA position 28223-29710
• Species: phage lambda
• Insert Size (bp): 1500
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 4

• Gene/Insert name: 2000 bp EcoRV fragment
• Alt name: lambda DNA position 36060-37994
• Species: phage lambda
• Insert Size (bp): 2000
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 5

• Gene/Insert name: 500 bp PstI fragment
• Alt name: lambda DNA position 28223-28663
• Species: phage lambda
• Insert Size (bp): 500
• GenBank ID: NC_001416.1

Gene/Insert 6

• Gene/Insert name: 700 bp PstI fragment
• Alt name: lambda DNA position 8783-9468
• Species: phage lambda
• Insert Size (bp): 700
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 6

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 7

• Gene/Insert name: 800 bp PstI fragment
• Alt name: G9a position 2757-3535
• Alt name: EHMT2
• Species: H. sapiens (human)
• Insert Size (bp): 800
• GenBank ID: NM_006709.4
• Entrez Gene: EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)

Cloning Information for Gene/Insert 7

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 8

• Gene/Insert name: 900 bp PstI fragment
• Alt name: lambda DNA position 143381-15280
• Species: phage lambda
• Insert Size (bp): 900
• GenBank ID: NC_001416.1

Cloning Information for Gene/Insert 8

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 9

• Gene/Insert name: 1000 bp PstI fragment
• Alt name: Bmi1 position 5-979
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: NM_005180.8
• Entrez Gene: BMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)

Gene/Insert 10

• Gene/Insert name: 2000 bp PstI fragment
• Alt name: lambda DNA position 28670-29710 and 36060-37994
• Species: phage lambda
• Insert Size (bp): 2000
• GenBank ID: NC_001416.1

Resource Information

• Supplemental Documents:
  • Preparation of Penn State DNA ladders
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry