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pAKTaq
(Plasmid #25712)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25712 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Taq polymerase
  • Species
    Thermus aquaticus
  • Insert Size (bp)
    2501

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer Tac promoter
  • 3′ sequencing primer M13_puc_F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKTaq was a gift from David Engelke (Addgene plasmid # 25712 ; http://n2t.net/addgene:25712 ; RRID:Addgene_25712)
  • For your References section:

    Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Engelke DR, Krikos A, Bruck ME, Ginsburg D. Anal Biochem. 1990 Dec . 191(2):396-400. 10.1016/0003-2697(90)90238-5 PubMed 2085185