1
Plasmid 25712: pAKTaq
  • Bacterial expression of Taq polymerase

  • Taq polymerase

  • 2501

  • Thermus aquaticus

  • pTTQ18
    (Search Vector Database)

  • Bacterial Expression

  • 4563

  • EcoRI

  • No

  • BglII

  • No

  • Tac promoter List of Sequencing Primers

  • M13_puc_F

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • View map

  • pAKTaq protocol (application/pdf)

  • David Engelke

  • MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Engelke et al (Anal Biochem. 1990 Dec . 191(2):396-400. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25712" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only