Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #25712)


Item Catalog # Description Quantity Price (USD)
Plasmid 25712 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Taq polymerase
  • Species
    Thermus aquaticus
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer Tac promoter
  • 3′ sequencing primer M13_puc_F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKTaq was a gift from David Engelke (Addgene plasmid # 25712 ; ; RRID:Addgene_25712)
  • For your References section:

    Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Engelke DR, Krikos A, Bruck ME, Ginsburg D. Anal Biochem. 1990 Dec . 191(2):396-400. 10.1016/0003-2697(90)90238-5 PubMed 2085185