Addgene - pAKTaq Plasmid Data

Plasmid 25712: pAKTaq

Gene/insert name:
Taq polymerase
Insert size:
2501
Species:
Thermus aquaticus
Vector backbone:
pTTQ18
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4563
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
Tac promoter List of Sequencing Primers
3' sequencing primer:
M13_puc_F
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pAKTaq protocol (application/pdf)
Principal Investigator:
David Engelke
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Engelke et al (Anal Biochem. 1990 Dec . 191(2):396-400. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 25712" in your Materials and Methods section.