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pPSU2
(Plasmid #89566)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89566 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC9
  • Backbone size w/o insert (bp) 2635
  • Total vector size (bp) 7750
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    750 bp EcoRV fragment
  • Alt name
    lambda DNA position 650-1399
  • Species
    phage lambda
  • Insert Size (bp)
    750
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    3000 bp EcoRV fragment
  • Alt name
    lambda DNA position 25345-26210
  • Species
    phage lambda
  • Insert Size (bp)
    3000
  • GenBank ID
    NC_001416.1

Gene/Insert 3

  • Gene/Insert name
    4000 bp EcoRV fragment
  • Alt name
    lambda DNA position 22948-24306
  • Species
    phage lambda
  • Insert Size (bp)
    4000
  • GenBank ID
    NC_001416.1

Gene/Insert 4

  • Gene/Insert name
    50 bp PstI fragment
  • Alt name
    lambda DNA position 26172-26210
  • Species
    phage lambda
  • Insert Size (bp)
    50
  • GenBank ID
    NC_001416.1

Gene/Insert 5

  • Gene/Insert name
    100 bp PstI fragment
  • Alt name
    lambda DNA position 2554-2656
  • Species
    phage lambda
  • Insert Size (bp)
    100
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 5

  • Cloning method Restriction Enzyme
  • 5′ cloning site NgoMIV (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 6

  • Gene/Insert name
    200 bp PstI fragment
  • Alt name
    lambda DNA 37262-37455
  • Species
    phage lambda
  • Insert Size (bp)
    200
  • GenBank ID
    NC_001416.1

Gene/Insert 7

  • Gene/Insert name
    300 bp PstI fragment
  • Alt name
    lambda DNA postion 3856-4149
  • Species
    phage lambda
  • Insert Size (bp)
    300
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 7

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site BsrGi (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 8

  • Gene/Insert name
    400 bp PstI fragment
  • Alt name
    lambda DNA position 19833-20232
  • Species
    phage lambda
  • Insert Size (bp)
    400
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 8

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site None (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 9

  • Gene/Insert name
    500 bp PstI fragment
  • Alt name
    lambda DNA position 11835-12334
  • Species
    phage lambda
  • Insert Size (bp)
    500
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 9

Gene/Insert 10

  • Gene/Insert name
    600 bp PstI fragment
  • Alt name
    lambda DNA position 2858-3447
  • Species
    phage lambda
  • Insert Size (bp)
    600
  • GenBank ID
    NC_001416.1

Cloning Information for Gene/Insert 10

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (not destroyed)
  • 3′ cloning site BsrGi (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Gene/Insert 11

  • Gene/Insert name
    1500 bp Psti fragment
  • Alt name
    lambda DNA position 25340-26170 and 743-1399
  • Species
    phage lambda
  • Insert Size (bp)
    1500
  • GenBank ID
    NC_001416.1

Gene/Insert 12

  • Gene/Insert name
    4100 bp PstI fragment
  • Alt name
    lambda DNA position 22948-24306
  • Species
    phage lambda
  • Insert Size (bp)
    4100
  • GenBank ID
    NC_001416.1

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSU2 was a gift from Song Tan (Addgene plasmid # 89566)
  • For your References section:

    The pPSU Plasmids for Generating DNA Molecular Weight Markers. Henrici RC, Pecen TJ, Johnston JL, Tan S. Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. 10.1038/s41598-017-02693-1 [pii] PubMed 28550309