GST-GGA3-VHS-GAT
(Plasmid
#173719)
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PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-5X-1
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Backbone manufacturerPharmacia Biotech
- Backbone size w/o insert (bp) 4972
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGGA3
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Alt namegolgi associated, gamma adaptin ear containing, ARF binding protein 3
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SpeciesH. sapiens (human)
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Insert Size (bp)939
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-GGA3-VHS-GAT was a gift from Juan Bonifacino (Addgene plasmid # 173719 ; http://n2t.net/addgene:173719 ; RRID:Addgene_173719) -
For your References section:
GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. Dell'Angelica EC, Puertollano R, Mullins C, Aguilar RC, Vargas JD, Hartnell LM, Bonifacino JS. J Cell Biol. 2000 Apr 3;149(1):81-94. doi: 10.1083/jcb.149.1.81. 10.1083/jcb.149.1.81 PubMed 10747089